Efficient Yeast One-/Two-Hybrid Screening Using a Library Composed Only of Transcription Factors in Arabidopsis thaliana
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منابع مشابه
Yeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
SETH4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of Arabidopsis thaliana. This fragment was PCR amplified using Kod Hi Fi DNA polymerase enzyme. This fragment was cloned into pGBKT7 bate vector and transformed E. coli DH5? cells containing vector were selected on LB medium containing Kanamycin. Finally, pGBKT7-SETH4 bate was transformed into yeast ...
متن کاملyeast two hybrid cdna screening of arabidopsis thaliana for seth4 protein interaction
seth4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of arabidopsis thaliana. this fragment was pcr amplified using kod hi fi dna polymerase enzyme. this fragment was cloned into pgbkt7 bate vector and transformed e. coli dh5? cells containing vector were selected on lb medium containing kanamycin. finally, pgbkt7-seth4 bate was transformed into yeast ...
متن کاملسنتز بانک cdna گیاه arabidopsis و غربالگری با روش yeast two hybrid جهت یافتن یک میانکنش مولکولی با پروتئین msi4
کشف شبکه های اینتراکتوم کلیدی برای درک سیستم های زیستی و در نتیجه حیات است. از این رو تکنیک های زیادی برای ردیابی تعامل های مولکولی و مکانیسم های عملکردی و فیزیولوژیکی ابداع شده اند. تکنیک دوبل هیبرید مخمر هم روشی برای درک میانکنش های پروتئینی می باشد این تکنیک بر مکانیسم رونویسی استوار است. ما از این روش برای درک مکانیسمی که در گذر از مرحله رویشی به زایشی دخالت دارد استفاده کردیم. آغاز گلدهی د...
15 صفحه اولDevelopment and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system
Protein-protein interaction (PPI) network maintains proper function of all organisms. Simple high-throughput technologies are desperately needed to delineate the landscape of PPI networks. While recent state-of-the-art yeast two-hybrid (Y2H) systems improved screening efficiency, either individual colony isolation, library preparation arrays, gene barcoding or massive sequencing are still requi...
متن کاملTwo bHLH Transcription Factors, bHLH34 and bHLH104, Regulate Iron Homeostasis in Arabidopsis thaliana.
The regulation of iron (Fe) homeostasis is critical for plant survival. Although the systems responsible for the reduction, uptake, and translocation of Fe have been described, the molecular mechanism by which plants sense Fe status and coordinate the expression of Fe deficiency-responsive genes is largely unknown. Here, we report that two basic helix-loop-helix-type transcription factors, bHLH...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 2010
ISSN: 1471-9053,0032-0781
DOI: 10.1093/pcp/pcq161